产品编号 ABIN934953
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for GrpE 蛋白 (ABIN934953)
抗原
GrpE
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 90 % pure
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序列
- MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
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产品特性
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Purified recombinant E.coli GrpE protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. -
内毒素水平
- < 1.0 EU per µg of protein (determined by LAL method)
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GrpE (AA 1-197), (V44I) protein
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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应用备注
- GrpE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Supplied as a liquid in 20 mM Tris, pH 8.0, containing 100 mM NaCl.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- GrpE
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背景
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GrpE ,co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.
Alternative Names: GrpE protein, HSP-70 cofactor protein, Heat shock protein B25.3 protein, HSP24 protein, HSP 70 cofactor. -
分子量
- 21.8 kDa (197 AA)
抗原
-
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