GrpE (AA 1-197), (V44I) 蛋白
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北京 101111
Quick Overview for GrpE (AA 1-197), (V44I) 蛋白 (ABIN667052)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-197, V44I
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序列
- MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
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产品特性
- GrpE, E.coli, Recombinant, E.coli
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纯化方法
- > 90% by SDS-PAGE
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 100 mM NaCl
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注意事项
- Avoid repeated freezing and thawing cycles
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- GrpE
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背景
- GrpE, co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.
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分子量
- 21.8 kDa (197aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_417104
抗原
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