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- 抗原 See all TXN 蛋白
- TXN (Thioredoxin (TXN))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
- 产品特性
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Purified recombinant Human Thioredoxin1 protein
Expression System: E.coli
Bioactivity: Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Molecular weight on SDS-PAGE will appear higher. - 纯度
- > 95 % pure
- Top Product
- Discover our top product TXN 蛋白
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Thioredoxin (TXN) protein
TXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Thioredoxin (TXN) (AA 1-105) (Active) proteinTXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Thioredoxin (TXN) (AA 2-109) protein (His tag)TXN 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS-PAGE SDS
Thioredoxin (TXN) (AA 1-105) protein (GST tag)TXN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Thioredoxin (TXN) (AA 1-105) (Active) protein (His tag)TXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Thioredoxin (TXN) (Active) proteinTXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % as determined by reducing SDS-PAGE. Active
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- 应用备注
- Thioredoxin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- 实验流程
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- Prepare a 1 mL reaction cocktail in a suitable container. The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
2. Add 4 µg of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25 °C for 20 minutes.
4. Record the increase in A650 nm for 15 minutes
- Prepare a 1 mL reaction cocktail in a suitable container. The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Supplied as a liquid in PBS, pH 7.4.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- RT/-20 °C
- 储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- 抗原
- TXN (Thioredoxin (TXN))
- 别名
- Thioredoxin1 (TXN 产品)
- 别名
- TRDX Protein, TRX Protein, TRX1 Protein, ADF Protein, AW550880 Protein, Trx1 Protein, Txn Protein, trx Protein, TXN Protein, SCM1.18 Protein, trxA3 Protein, thioredoxin Protein, TR-1 Protein, Trx-1 Protein, thioredoxin Protein, thioredoxin 1 Protein, thioredoxin L homeolog Protein, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Protein, thioredoxin pseudogene Protein, cytosolic thioredoxin Trx1 Protein, TXN Protein, Txn1 Protein, txn.L Protein, txn Protein, trx Protein, SCO0885 Protein, PHG25ORF099c Protein, LOC100349046 Protein, Txn Protein, trx1 Protein
- 背景
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Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: ATL derived factor protein, TXN protein protein, Thioredoxin 1 protein, TR-I protein, TRX1 protein, TXN protein, TRX protein, , DKFZp686B1993 protein, Thioredoxin1, Surface associated sulphydryl protein protein, Thioredoxin protein, Thioredoxin -1 protein, Thioredoxin I protein, TRX 1 protein, Thioredoxin-2 protein, Thioredoxin-1 protein, ADF protein, SASP protein, Thioredoxin 1, TRDX protein, TRX2 protein, MGC61975 protein, Thioredoxin -1, Thioredoxin-1 protein - 分子量
- 11.7 kDa (105 AA)
- 途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
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