Quick Overview for TXN 蛋白 (ABIN934926)
抗原
See all TXN 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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序列
- MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
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产品特性
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Purified recombinant Human Thioredoxin1 protein
Expression System: E.coli
Bioactivity: Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Molecular weight on SDS-PAGE will appear higher.
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Thioredoxin (TXN) (AA 1-105) protein (GST tag)
TXN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Thioredoxin (TXN) proteinTXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Thioredoxin (TXN) (Active) proteinTXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % as determined by reducing SDS-PAGE. Active
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应用备注
- Thioredoxin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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实验流程
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- Prepare a 1 mL reaction cocktail in a suitable container. The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
2. Add 4 µg of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25 °C for 20 minutes.
4. Record the increase in A650 nm for 15 minutes
- Prepare a 1 mL reaction cocktail in a suitable container. The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Supplied as a liquid in PBS, pH 7.4.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- TXN (Thioredoxin (TXN))
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别名
- Thioredoxin1
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背景
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Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: ATL derived factor protein, TXN protein protein, Thioredoxin 1 protein, TR-I protein, TRX1 protein, TXN protein, TRX protein, , DKFZp686B1993 protein, Thioredoxin1, Surface associated sulphydryl protein protein, Thioredoxin protein, Thioredoxin -1 protein, Thioredoxin I protein, TRX 1 protein, Thioredoxin-2 protein, Thioredoxin-1 protein, ADF protein, SASP protein, Thioredoxin 1, TRDX protein, TRX2 protein, MGC61975 protein, Thioredoxin -1, Thioredoxin-1 protein -
分子量
- 11.7 kDa (105 AA)
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途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
抗原
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