GLB1 蛋白
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- 抗原 See all GLB1 蛋白
- GLB1 (Galactosidase, beta 1 (GLB1))
- 蛋白类型
- Recombinant
- 适用
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- 宿主
- 请咨询
- 应用范围
- Immunoassay (IA)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 过滤
- Sterile filtered
- Top Product
- Discover our top product GLB1 蛋白
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Galactosidase, beta 1 (GLB1) protein (Fc Tag)
GLB1 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE.
Galactosidase, beta 1 (GLB1) (AA 578-677) protein (GST tag)GLB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Galactosidase, beta 1 (GLB1) (AA 1-677) protein (GST tag)GLB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Galactosidase, beta 1 (GLB1) (AA 29-677) protein (His tag)Crystallography grade GLB1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Galactosidase, beta 1 (GLB1) (AA 30-647) protein (His tag)Crystallography grade GLB1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Beta Galactosidase is used as a control in beta-galactosidase based immunological assays.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.05 M Potassium Phosphate, pH 7.8
- 储存条件
- 4 °C
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- 抗原
- GLB1 (Galactosidase, beta 1 (GLB1))
- 别名
- Beta Galactosidase (GLB1 产品)
- 别名
- EBP Protein, ELNR1 Protein, MPS4B Protein, DER Protein, XR Protein, 0610038K04Rik Protein, 1810027P18Rik Protein, glb Protein, CG9092 Protein, Dmel\\CG9092 Protein, beta-GAL Protein, beta-Gal-1 Protein, beta-gal Protein, gal Protein, lacZ-1 Protein, AW125515 Protein, Bge Protein, Bgl Protein, Bgl-e Protein, Bgl-s Protein, Bgl-t Protein, Bgs Protein, Bgt Protein, C130097A14Rik Protein, BGAL Protein, zgc:110823 Protein, ECK0341 Protein, JW0335 Protein, GLB Protein, galactosidase beta 1 Protein, dicarbonyl and L-xylulose reductase Protein, dicarbonyl L-xylulose reductase Protein, beta-D-galactosidase Protein, beta-galactosidase Protein, beta galactosidase Protein, galactosidase, beta 1 Protein, beta-galactosidase-like Protein, GLB1 Protein, DCXR Protein, Dcxr Protein, lacZ Protein, SSP_RS00525 Protein, lacH Protein, ECL_03691 Protein, Gal Protein, Glb1 Protein, glb1 Protein, VV2_1327 Protein, LOC100056371 Protein
- 背景
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β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. Lactase is often confused as an alternative name for β-galactosidase, but it is actually simply a sub-class of β-galactosidase. β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. It is an essential enzyme in the human body, deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. It is commonly used in molecular biology as a reporter marker to monitor gene expression. It also exhibits a phenomenon called α-complementation which forms the basis for the blue/white screening of recombinant clones. This enzyme can be split in two peptides, LacZα and LacZΩ, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors where the presence of the lacZα gene in a plasmid can complement in trans another mutant gene encoding the LacZΩ in specific laboratory strains of E. coli. However, when DNA fragments are inserted in the vector, the production of LacZα is disrupted, the cells therefore show no β-galactosidase activity. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.
Synonyms: GLB1 - UniProt
- P16278
- 途径
- Glycosaminoglycan Metabolic Process
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