PKC iota 蛋白
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北京 101111
Quick Overview for PKC iota 蛋白 (ABIN7939472)
抗原
See all PKC iota (PRKCI) 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant PRKCI protein
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Protein Kinase C, iota (PRKCI) (AA 1-587) protein (GST tag)
PRKCI 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA, KAA
Protein Kinase C, iota (PRKCI) protein (Myc-DYKDDDDK Tag)PRKCI 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant PRKCI protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
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别名
- PRKCI
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背景
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Protein Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCI (Protein Kinase C Iota), a calcium- and diacylglycerol-independent serine/threonine-protein kinase that plays a general protective role against apoptotic stimuli and is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. PRKCI is necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. In cultured neurons, PRKCI prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, PRKCI may function downstream of phosphatidylinositol 3-kinase (PI(3) K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. PRKCI can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway.
Short Description: Recombinant PRKCI protein was expressed in baculovirus expression system as the full-length protein (accession number NP_002731.4) with a N-terminal FLAG tag. The molecular weight of the protein is 69.5 kDa.
Aliases: Protein Kinase C lota, PKC lota
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途径
- Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation
抗原
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