Nemo-Like Kinase Protein (NLK)
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Quick Overview for Nemo-Like Kinase Protein (NLK) (ABIN7939365)
抗原
See all Nemo-Like Kinase (NLK) 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant NLK protein
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Nemo-Like Kinase (NLK) (AA 1-515) protein (GST tag)
NLK 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA, KAA
Nemo-Like Kinase (NLK) (Active) protein (GST tag,His tag)NLK 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 91 % as determined by reducing SDS-PAGE. Active
Nemo-Like Kinase (NLK) protein (Myc-DYKDDDDK Tag)NLK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant NLK protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- Nemo-Like Kinase (NLK)
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别名
- NLK
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背景
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Protein Background: NLK (Nemo Like Kinase), a serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 and negative regulator of the canonical Wnt/beta-catenin signaling pathway. NLK binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. NLK acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Diseases associated with NLK include Diamond-Blackfan Anemia.
Short Description: Recombinant NLK protein was expressed in baculovirus expression system as the full length protein (accession number NP_057315.3) with a N-terminal FLAG tag. The molecular weight of the protein is 59.5 kDa.
Aliases: Nemo Like Kinase
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途径
- Ubiquitin Proteasome Pathway
抗原
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