Nemo-Like Kinase Protein (NLK) (His-GST)
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Quick Overview for Nemo-Like Kinase Protein (NLK) (His-GST) (ABIN7317069)
抗原
See all Nemo-Like Kinase (NLK) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Nemo-Like Kinase protein is labelled with His-GST.
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原理
- Recombinant Human NLK Protein (His & GST Tag)
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序列
- Val121-Glu 527
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产品特性
- A DNA sequence encoding the human NLK (Q9UBE8) (Val121-Glu527) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.
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Nemo-Like Kinase (NLK) (AA 1-515) protein (GST tag)
NLK 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA, KAA
Nemo-Like Kinase (NLK) protein (Myc-DYKDDDDK Tag)NLK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- Nemo-Like Kinase (NLK)
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别名
- NLK
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背景
- LAK1,NLK,Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.
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分子量
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Calculated MW: 74.1 kDa
Observed MW: 73 kDa
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基因ID
- 51701
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UniProt
- Q9UBE8
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途径
- Ubiquitin Proteasome Pathway
抗原
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