ITK Protein (AA 352-620)
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Quick Overview for ITK Protein (AA 352-620) (ABIN7939168)
抗原
See all ITK 蛋白蛋白类型
宿主
资源
应用范围
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产品特性
- AA 352-620
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原理
- Recombinant ITK (352-620) protein
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 152,621.24 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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IL2-Inducible T-Cell Kinase (ITK) (AA 1-620) protein (His tag)
custom-made ITK 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
IL2-Inducible T-Cell Kinase (ITK) (AA 1-620) protein (GST tag)ITK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
IL2-Inducible T-Cell Kinase (ITK) (AA 147-251) protein (His-IF2DI Tag)ITK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant ITK (352-620) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- ITK (IL2-Inducible T-Cell Kinase (ITK))
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别名
- ITK
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背景
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Protein Background: ITK (IL2 Inducible T Cell Kinase) is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response and regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. The phosphorylation leads to ITK autophosphorylation and full activation. Once activated, it phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. It also phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Diseases associated with ITK include Lymphoproliferative Syndrome 1 and Agammaglobulinemia, X-Linked.
Short Description: Recombinant ITK (352-620) protein that includes amino acids 352-620 of human ITK protein (accession number NP_005537.3) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 31.8 kDa.
Aliases: IL-2 Inducible T-Cell Kinase, Tyrosine-Protein Kinase LYK, EMT, LYK, LPFS1
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
抗原
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