ITK Protein (AA 1-620) (His tag)
This Recombinant ITK protein is expressed in HEK-293 Cells.
产品编号 ABIN7554214
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for ITK Protein (AA 1-620) (His tag) (ABIN7554214)
抗原
See all ITK 蛋白
ITK
(IL2-Inducible T-Cell Kinase (ITK))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
质量等级
custom-made
-
-
产品特性
- AA 1-620
-
标记
- This ITK protein is labelled with His tag.
-
原理
- Predefined custom protein recombinant ITK Protein expressed in mammalian cells.
-
序列
- MNNFILLEEQ LIKKSQQKRR TSPSNFKVRF FVLTKASLAY FEDRHGKKRT LKGSIELSRI KCVEIVKSDI SIPCHYKYPF QVVHDNYLLY VFAPDRESRQ RWVLALKEET RNNNSLVPKY HPNFWMDGKW RCCSQLEKLA TGCAQYDPTK NASKKPLPPT PEDNRRPLWE PEETVVIALY DYQTNDPQEL ALRRNEEYCL LDSSEIHWWR VQDRNGHEGY VPSSYLVEKS PNNLETYEWY NKSISRDKAE KLLLDTGKEG AFMVRDSRTA GTYTVSVFTK AVVSENNPCI KHYHIKETND NPKRYYVAEK YVFDSIPLLI NYHQHNGGGL VTRLRYPVCF GRQKAPVTAG LRYGKWVIDP SELTFVQEIG SGQFGLVHLG YWLNKDKVAI KTIREGAMSE EDFIEEAEVM MKLSHPKLVQ LYGVCLEQAP ICLVFEFMEH GCLSDYLRTQ RGLFAAETLL GMCLDVCEGM AYLEEACVIH RDLAARNCLV GENQVIKVSD FGMTRFVLDD QYTSSTGTKF PVKWASPEVF SFSRYSSKSD VWSFGVLMWE VFSEGKIPYE NRSNSEVVED ISTGFRLYKP RLASTHVYQI MNHCWKERPE DRPAFSRLLR QLAEIAESGL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
-
特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
-
产品特性
-
Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
-
-
想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Wheat germConjugate GST tagOrigin HumanPrice 5,782.24 ¥表达系统 Escherichia coli (E. coli)Conjugate His-IF2DI TagOrigin HumanPrice 3,889.02 ¥表达系统 Baculovirus infected Insect CellsConjugate GST tagOrigin HumanPrice 4,043.02 ¥表达系统 Baculovirus infected Insect CellsConjugate GST tagOrigin HumanPrice 4,043.02 ¥表达系统 Insect CellsConjugate GST tagOrigin HumanPrice 4,054.05 ¥表达系统 HEK-293 CellsConjugate Myc-DYKDDDDK TagOrigin HumanPrice 10,600.77 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin Human, Mouse, RatPrice 3,136.30 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
IL2-Inducible T-Cell Kinase (ITK) (AA 1-620) protein (GST tag)
ITK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
IL2-Inducible T-Cell Kinase (ITK) (AA 147-251) protein (His-IF2DI Tag)ITK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
IL2-Inducible T-Cell Kinase (ITK) protein (Myc-DYKDDDDK Tag)ITK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- The buffer composition is at the discretion of the manufacturer.
-
注意事项
- Avoid repeated freeze-thaw cycles.
-
储存条件
- -80 °C
-
储存方法
- Store at -80°C.
-
有效期
- 12 months
-
-
- ITK (IL2-Inducible T-Cell Kinase (ITK))
-
别名
- ITK
-
背景
- Tyrosine-protein kinase ITK/TSK (EC 2.7.10.2) (Interleukin-2-inducible T-cell kinase) (IL-2-inducible T-cell kinase) (Kinase EMT) (T-cell-specific kinase) (Tyrosine-protein kinase Lyk),FUNCTION: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed:12186560, PubMed:12682224, PubMed:21725281). Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells (By similarity). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity). {ECO:0000250|UniProtKB:Q03526, ECO:0000269|PubMed:12186560, ECO:0000269|PubMed:12682224, ECO:0000269|PubMed:21725281}.
-
分子量
- 71.8 kDa
-
UniProt
- Q08881
-
AlphaFold
- Q08881
-
途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
抗原
-
You are here:
