GRK5 蛋白
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Quick Overview for GRK5 蛋白 (ABIN7938854)
抗原
See all GRK5 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant GRK5 protein
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G Protein-Coupled Receptor Kinase 5 (GRK5) protein (Myc-DYKDDDDK Tag)
GRK5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant GRK5 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))
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别名
- GRK5
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背景
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Protein Background: GRK5 is a serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. GRK5 phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, it also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Diseases associated with GRK5 include Systolic Heart Failure and Palmoplantar Keratoderma and Congenital Alopecia 2.
Short Description: Recombinant GRK5 protein was expressed in baculovirus expression system as the full length protein (accession number NP_005299.1) with a N-terminal FLAG tag. The molecular weight of the protein is 68.8 kDa.
Aliases: G-protein Receptor Kinase 5
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途径
- Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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