BRD4 蛋白
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北京 101111
Quick Overview for BRD4 蛋白 (ABIN7938640)
抗原
See all BRD4 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant BRD4 protein
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Bromodomain Containing 4 (BRD4) protein
BRD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Bromodomain Containing 4 (BRD4) (AA 1-1362) protein (Fc Tag)BRD4 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
Bromodomain Containing 4 (BRD4) (AA 49-170) protein (His tag)BRD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Bromodomain Containing 4 (BRD4) (AA 44-168) protein (His tag,DYKDDDDK Tag)BRD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant ScA, AcA, Func
Bromodomain Containing 4 (BRD4) (AA 333-460) protein (His tag,DYKDDDDK Tag)BRD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >87 % pure by SDS-PAGE ScA, AcA, Func
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant BRD4 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- BRD4 (Bromodomain Containing 4 (BRD4))
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别名
- BRD4
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背景
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Protein Background: Bromodomain-containing protein 4 (BRD4) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female sterile homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD4 has been identified recently as a therapeutic target in many cancers, including acute myeloid leukemia, multiple myeloma, Burkitt's lymphoma, NUT midline carcinoma, colon cancer, and breast cancer. BRD4 regulates the transcription of oncogenes, HIV, and human papilloma virus (HPV). It has been shown to bind and phosphorylate RNA pol II, which implicates its involvement in the regulation of eukaryotic transcription. It shows binding specificity for acetylated H3K9, H3K9/K14, H4K5, H4K8, H4K12, H4K5/K8, H4K5/K12, H4K8/K12, H4K12/K16, H4K12/K16/K20 and H4K5/K8/K12/K16, as well as acetylated RelA-K310.
Short Description: Recombinant BRD4 protein that includes full length of human BRD4 protein (accession number NP_055114.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 81.7 kDa.
Aliases: Bromodomain containing protein 4, HUNK1, FSHRG4, HUNKI, MCAP, CAP
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途径
- Chromatin Binding, SARS-CoV-2 Protein Interactome
抗原
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