BDNF Protein (AA 129-247)
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北京 101111
Quick Overview for BDNF Protein (AA 129-247) (ABIN7937438)
抗原
See all BDNF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 129-247
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原理
- Recombinant Human/Murine/Rat/Canine/Equine Brain-derived Neurotrophic Factor
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 EU/ug of rHu/Mu/Rt/Ca/EqBDNF as determined by LAL method.
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Biological Activity Comment
- Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of rHu/Mu/Rt/Ca/EqBDNF is approximately 1.3 x 103units/ug, which is calibrated against recombinant human BDNF WHO Standard. Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera is coated at 1 ug/mL, rHu/Mu/Rt/Ca/EqBDNF binds with an apparent Kd <1 nM.
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说明
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Apparent molecular mass of 13-14 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 119 amino acids.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from 0.2 um filtered concentrated solution in 100 mM Sodium Citrate and 300 mM NaCl, pH 3.0.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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有效期
- 12 months
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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别名
- Brain-derived Neurotrophic Factor
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分子量
- 14 kDa
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UniProt
- P23560
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途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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