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NOG 蛋白

This Recombinant NOG protein is expressed in Insect Cells.
产品编号 ABIN7937428
发货至: 中国
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Quick Overview for NOG 蛋白 (ABIN7937428)

抗原

See all NOG 蛋白
NOG (Noggin (NOG))

蛋白类型

Recombinant

生物活性

Active

宿主

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资源

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Insect Cells

纯度

> 95 % by SDS-PAGE and HPLC analyses.
  • 原理

    Recombinant Human Noggin, Insect Cells Derived

    序列

    QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC

    过滤

    0.2 μm filtered

    内毒素水平

    Less than 0.1 EU/ug of rHuNoggin, Insect Cell as determined by LAL method.

    Biological Activity Comment

    Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.
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  • 说明

    Approximately 23.0 kDa on SDS-PAGE under reducing conditions, containing 205 amino acids, and a molecular mass about 47.9 kDa homodimer under non-reducing conditions (Molecular size on SDS-PAGE will appear at approximately 50-80 kDa).

    限制

    仅限研究用
  • 状态

    Lyophilized

    缓冲液

    Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5 % trehalose, 0.02 %Tween-20.

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    有效期

    12 months
  • 抗原

    NOG (Noggin (NOG))

    别名

    Noggin

    分子量

    23.0 kDa,47.9 kDa,80 kDa

    基因ID

    9241

    UniProt

    Q13253

    途径

    Stem Cell Maintenance, Tube Formation
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