VEGFA 蛋白
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北京 101111
Quick Overview for VEGFA 蛋白 (ABIN7936832)
抗原
See all VEGFA 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast
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序列
- MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 EU/ug of rRtVEGF164, Yeast as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/ml.
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说明
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Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
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储存条件
- -20 °C
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储存方法
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
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有效期
- 12 months
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- Vascular Endothelial Growth Factor 164
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分子量
- 25.7 kDa
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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