FGF2 蛋白
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北京 101111
Quick Overview for FGF2 蛋白 (ABIN7866452)
抗原
See all FGF2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Recombinant Human FGF-2 (basic) Protein
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序列
- AGSITTLPAL PEDGGSGAFP PGHFKDPKRL YCKNGGFFLR IHPDGRVDGV REKSDPHIKL QLQAEERGVV SIKGVCANRY LAMKEDGRLL ASKCVTDECF FFERLESNNY NTYRSRKYTS WYVALKRTGQ YKLGSKTGPG QKAILFLPMS AKS
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特异性
- Chromosomal location:4q26
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产品特性
- Length (aa):153
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内毒素水平
- < 0.1 ng per μg of human FGF-2
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Fibroblast Growth Factor 2 (Basic) (FGF2) (full length) (Active) protein
FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE CC Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 135-288), (Isoform 1) (Active) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (AVI tag,His tag,Biotin)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) protein (GST tag)FGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (His tag)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 9-154) (Active) protein (His tag)FGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 10-154) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- The ED50 for stimulation of cell proliferation in HUVECs by human FGF-2 (basic) has been determined to be in the range of 0.1-2 ng/mL. The WHO standard #90/712 was used as control.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- water,The lyophilized FGF-2 (basic) should be reconstituted in water to a concentration not lower than 50 μg/mL. For long term storage we would recommend to add at least 0.1 % human or bovine serum albumin.
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缓冲液
- PBS
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储存条件
- -20 °C,-80 °C
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储存方法
- Lyophilized samples are stable for greater than six months at -20°C to -70°C.
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有效期
- 6 months
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- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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别名
- FGF-2
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背景
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Synonyms: FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2
Description: FGF basic (FGF2, HBGF2) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60 % amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted. Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH3T3 cells. The 17 kDa mouse sequence has 98 % aa identity with rat, and 95 % identity with human, bovine and sheep FGF basic. Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.
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分子量
- 17.0 kDa
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基因ID
- 2247
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NCBI登录号
- NM_002006, NP_001997
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UniProt
- P09038
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
抗原
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