WISP1 Protein (AA 23-367)
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北京 101111
Quick Overview for WISP1 Protein (AA 23-367) (ABIN7866102)
抗原
See all WISP1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 23-367
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原理
- Human WISP1 Protein Recombinant
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序列
- TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN
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产品特性
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Protein Size: Recombinant WISP1 migrates as an approximately 38 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human WISP1 mature chain including a C-terminal 6xHis tag was expressed in E. Coli. Recombinant Human WNT1-inducible-signaling pathway protein 1 encodes amino acid residues 23-367.
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内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 μg/ml.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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缓冲液
- WISP1 was Lyophilized from PBS, pH 7.2.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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有效期
- 24 months
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- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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别名
- WISP1
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分子量
- 38 kDa
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途径
- WNT signaling, Growth Factor Binding
抗原
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