MANF Protein (AA 25-182)
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北京 101111
Quick Overview for MANF Protein (AA 25-182) (ABIN7866035)
抗原
See all MANF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 25-182
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原理
- Human MANF Protein Recombinant
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序列
- LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
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产品特性
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Protein Size: Recombinant MANF migrates as an approximately 17 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human MANF mature chain including a C-terminal 6xHis tag was expressed in E. Coli. Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor encodes amino acid residues 25-182.
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内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml.
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Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (Active) protein
MANF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 1-182) protein (GST tag)MANF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) protein (His tag)MANF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) protein (His tag)MANF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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缓冲液
- MANF was Lyophilized from PBS, pH 7.2.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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有效期
- 24 months
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- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
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别名
- MANF
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分子量
- 17 kDa
抗原
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