HMGB1 Protein (BoxA 2D)
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北京 101111
Quick Overview for HMGB1 Protein (BoxA 2D) (ABIN7862613)
抗原
See all HMGB1 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- BoxA 2D
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原理
- BoxA 2D (negative control of BoxA wt), LPS-free
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序列
- BoxA 2D from HMGB1 consists of 89 amino acids: MGKGDPKKPR GKMSSYAFFV QTDREEHKKK HPDASVNFSE FSKKDSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF
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产品特性
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BoxA 2D is the mutant form of BoxA (DNA binding domains of HMGB1 protein) in which the two cysteines are replaced with aspartic acid.
It consists of 89 amino acids and has a calculated molecular mass of approximately 10.4 kDa.
BoxA 2D in migration assay cannot block the HMGB1 induced migration of fibroblasts.
It contains only trace amounts of LPS (<0.4 ng/mg protein).
BoxA 2D variant can be used as negative control of BoxA wt. -
纯化方法
- Purified
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内毒素水平
- LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) (Active) protein (Biotin,His tag)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
High Mobility Group Box 1 (HMGB1) (Fully reduced) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (C106S), (C23S), (C45S), (Non-oxidizable) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 宿主: 人 宿主: Baculovirus Recombinant > 90 % pure SDS
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl.
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缓冲液
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The protein is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request. -
储存条件
- 4 °C/-20 °C
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储存方法
- 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- High-mobility group protein B1 (HMGB1)
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背景
- HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.
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分子量
- 10.4 kDa
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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