Vinculin Protein (VCL) (AA 1-650) (His tag)
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Quick Overview for Vinculin Protein (VCL) (AA 1-650) (His tag) (ABIN7669965)
抗原
See all Vinculin (VCL) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-650
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标记
- This Vinculin protein is labelled with His tag.
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原理
- Recombinant Human Vinculin/VCL Protein (His Tag)
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序列
- Met1-Val650
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Vinculin (VCL) (AA 1-1066) protein (GST tag)
VCL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vinculin (VCL) (AA 777-1066) protein (GST tag)VCL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vinculin (VCL) (AA 2-235) protein (His-IF2DI Tag)VCL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vinculin (VCL) (AA 1-1066) protein (His tag)VCL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Vinculin (VCL) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)VCL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Vinculin (VCL)
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别名
- Vinculin,VCL
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背景
- CMD1W,CMH15,HEL114,MV,MVCL,Metavinculin,VCL,Vinculin,Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/α-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction(s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.
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分子量
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Calculated MW: 71.4 kDa
Observed MW: 60 kDa
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基因ID
- 7414
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UniProt
- P18206
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途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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