Ubiquitin Protein (Ub) (AA 103-229) (His tag)
This Recombinant Ubiquitin protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN7669930
发货至:
中国
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Quick Overview for Ubiquitin Protein (Ub) (AA 103-229) (His tag) (ABIN7669930)
抗原
See all Ubiquitin (Ub) 蛋白
Ubiquitin (Ub)
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Immunogen (Imm)
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 103-229
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标记
- This Ubiquitin protein is labelled with His tag.
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原理
- Recombinant Human Ub Protein (His Tag)
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序列
- Lys103-Cys229
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Ubiquitin (Ub) protein
Ub 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
Ubiquitin (Ub) (AA 1-76) proteinUb 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% SDS, WB, ELISA, IP
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Ubiquitin (Ub)
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别名
- Ub
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背景
- Polyubiquitin-B,UBB,Ubiquitin,Ubiquitin B,Polyubiquitin-B(UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.
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分子量
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Calculated MW: 13.9 kDa
Observed MW: 15 kDa
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基因ID
- 7314
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UniProt
- P0CG47
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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