Ubiquitin Protein (Ub) (AA 1-76)
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Quick Overview for Ubiquitin Protein (Ub) (AA 1-76) (ABIN7939666)
抗原
See all Ubiquitin (Ub) 蛋白蛋白类型
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资源
应用范围
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产品特性
- AA 1-76
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原理
- Recombinant Ubiquitin (1-76) protein
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant Ubiquitin (1-76) protein is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- Ubiquitin (Ub)
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别名
- Ubiquitin
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背景
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Protein Background: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Short Description: Recombinant Ubiquitin (1-76) protein that includes amino acids 1-76 of human UBC protein (accession number NP_066289.3) was expressed in E. coli and without any tag with a molecular weight of 8.56 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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