Renin Protein (REN) (His tag)
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Quick Overview for Renin Protein (REN) (His tag) (ABIN7669527)
抗原
See all Renin (REN) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Renin protein is labelled with His tag.
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原理
- Recombinant Human Renin Protein (His Tag)
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序列
- Leu67-406Arg
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Renin (REN) (AA 24-406) protein (GST tag)
REN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Renin (REN) (AA 24-406) protein (His tag)REN 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Renin (REN) (AA 22-402) protein (His tag)REN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Renin (REN) (Active) protein (His tag)REN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by reducing SDS-PAGE. ELISA Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Renin (REN)
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别名
- Renin
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背景
- Angiotensin forming enzyme,HNFJ2,REN,Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
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分子量
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Calculated MW: 37.3 kDa
Observed MW: 40 kDa
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基因ID
- 5972
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UniProt
- P00797
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
抗原
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