RAC1 Protein (AA 1-189) (His tag)
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Quick Overview for RAC1 Protein (AA 1-189) (His tag) (ABIN7669520)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-189
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标记
- This RAC1 protein is labelled with His tag.
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原理
- Recombinant Human Rac1 Protein(His Tag)
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序列
- Met1-Cys189
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纯化方法
- > 90 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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RAC1 (AA 1-192) protein (GST tag)
宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
RAC1 (AA 1-192) protein (His tag)宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
RAC1 protein (Myc-DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- RAC1
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背景
- Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1,Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly, In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity. In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization.
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分子量
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Calculated MW: 21.1 kDa
Observed MW: 27 kDa
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基因ID
- 5879
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UniProt
- P63000
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途径
- WNT signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling
抗原
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