LacI (AA 1-360) protein (His tag)
This Recombinant LacI protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN7669193
发货至:
中国
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Quick Overview for LacI (AA 1-360) protein (His tag) (ABIN7669193)
抗原
LacI
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
Immunogen (Imm)
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 1-360
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标记
- His tag
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原理
- Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)
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序列
- Met1-Gln360
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- LacI
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背景
- LacI,Lactose operon repressor,Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
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分子量
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Calculated MW: 39.4 kDa
Observed MW: 40 kDa
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UniProt
- P03023
抗原
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