LacI (AA 1-360) protein (His tag)
This Recombinant LacI protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN1692437
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for LacI (AA 1-360) protein (His tag) (ABIN1692437)
抗原
LacI
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 1-360
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标记
- His tag
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原理
- Recombinant E.coli LacI (C-6His)
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序列
- MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH
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产品特性
- Recombinant E.coli Lactose operon repressor is produced by our E.coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as a 0.2 um filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 5 mM DTT pH 8.0 .
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- minimize freeze-thaw cycles
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- LacI
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Sub Type
- Fusionprotein
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背景
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Alternative Names: Lactose operon repressor, LacILactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Alternative Names: Lactose operon repressor, LacI -
分子量
- 39.4 kDa
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UniProt
- P03023
抗原
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