IgG3 Protein (AA 97-329)
This Recombinant IgG3 protein is expressed in HEK-293 Cells.
Quick Overview for IgG3 Protein (AA 97-329) (ABIN7668927)
抗原
See all IgG3 蛋白
IgG3
蛋白类型
Recombinant
生物活性
Inactive
宿主
小鼠
资源
HEK-293 Cells
纯度
> 95 %
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产品特性
- AA 97-329
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原理
- Recombinant Mouse IgG3-Fc Protein
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序列
- Glu97-Lys329
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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IgG3 (AA 1-521) protein (GST tag)
宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- IgG3
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物质类
- Antibody
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背景
- As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.,Ig gamma-3 chain C region;IgG3 Fc
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分子量
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calculated_mw: 26.2 kDa
observed_mw: 31 kDa
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UniProt
- P03987
抗原
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