CD5 Protein (CD5) (His tag)
Quick Overview for CD5 Protein (CD5) (His tag) (ABIN7668385)
抗原
See all CD5 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This CD5 protein is labelled with His tag.
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原理
- Recombinant Rat CD5 / Cluster of Differentiation 5 protein (His tag)
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序列
- Ser 25-Pro 368
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD5
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别名
- CD5
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背景
- The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.,Cd5,T-cell surface glycoprotein CD5
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分子量
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calculated_mw: 37.7 kDa
observed_mw: 42 kDa
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UniProt
- P51882
抗原
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