ADGRE5 (AA 21-530) protein (Fc Tag)
This Recombinant ADGRE5 protein is expressed in Mammalian Cells.
产品编号 ABIN7668119
发货至:
中国
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Contact Info
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中国
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北京 101111
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Quick Overview for ADGRE5 (AA 21-530) protein (Fc Tag) (ABIN7668119)
抗原
ADGRE5
蛋白类型
Recombinant
宿主
人
资源
Mammalian Cells
应用范围
Immunogen (Imm)
纯度
> 90 % as determined by reducing SDS-PAGE.
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产品特性
- AA 21-530
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标记
- Fc Tag
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原理
- Recombinant Human ADGRE5 Protein( Fc Tag)
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序列
- Gln21-Leu530
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纯化方法
- SDS-PAGE analysis of Human ADGRE5 proteins, 2 μg/lane of Recombinant Human ADGRE5 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 80.9 KD
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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ADGRE5 protein (DYKDDDDK Tag)
宿主: 人 宿主: HEK-293 Cells Synthetic Nanodisc Imm, cryo-EM, ELISA, PhD, SPR
ADGRE5 (AA 21-552) protein (His tag)宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- ADGRE5
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背景
- ADGRE5,Adhesion G protein-coupled receptor E5,CD 97,CD_antigen=CD97,CD97,CD97 antigen,CD97 antigen subunit beta,CD97 Molecule,CD97,Leukocyte antigen CD97,Seven span transmembrane protein,Seven transmembrane helix receptor,Seven transmembrane heterodimeric receptor associated with inflammation,TM7LN 1,TM7LN1,The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium-binding site. Mature CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adhere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isoforms that are related to the calcium-binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.
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分子量
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Calculated MW: 80.9 kDa
Observed MW: 100-110 kDa
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基因ID
- 976
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UniProt
- P48960
抗原
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