14-3-3 sigma/SFN Protein (AA 1-248)
Quick Overview for 14-3-3 sigma/SFN Protein (AA 1-248) (ABIN7665627)
抗原
See all 14-3-3 sigma/SFN (SFN) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-248
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原理
- Recombinant Human Stratifin
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内毒素水平
- < 1 EU/μg as determined by LAL test.
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Stratifin (SFN) (AA 1-248) protein (His tag)
custom-made SFN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stratifin (SFN) (AA 1-248) protein (His tag)custom-made SFN 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stratifin (SFN) (AA 1-248) protein (GST tag)SFN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Stratifin (SFN) (AA 1-248), (full length) protein (GST tag)SFN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Stratifin (SFN) proteinSFN 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.4.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- The lyophilized protein is stable at -20°C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80°C for 3 months or 1 week at 2 to 8°C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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有效期
- 12 months
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- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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别名
- Stratifin
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背景
- 14-3-3 Protein Sigma, Epithelial Cell Marker Protein 1, Stratifin, SFN, HME1
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分子量
- 30 kDa
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UniProt
- P31947
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途径
- p53 Pathway, Myometrial Relaxation and Contraction
抗原
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