Protein A Protein (His tag)
This Recombinant Protein A protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for Protein A Protein (His tag) (ABIN7665109)
抗原
See all Protein A 蛋白
Protein A
蛋白类型
Recombinant
宿主
Staphylococcus aureus
资源
大肠杆菌（E. Coli）
应用范围
ELISA, Western Blotting (WB)
纯度
Greater than 90 % as determined by SDS-PAGE.
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标记
- This Protein A protein is labelled with His tag.
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原理
- Recombinant ProA-B4
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Protein A (AA 37-469) protein
宿主: Staphylococcus aureus 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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有效期
- 12 months
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- Protein A
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背景
- Protein A
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分子量
- 28.3 kDa
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UniProt
- P38507
抗原
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