IL-21 Protein (AA 18-146)
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北京 101111
Quick Overview for IL-21 Protein (AA 18-146) (ABIN7677341)
抗原
See all IL-21 (IL21) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 18-146
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序列
- MHKSSFQEQD LLLIRMRQLI DIVDQLKNYV NDLDPESLPA PEDVKRHCER SAFSCFQKVQ LKAANTGGNE QIINVLTKQL KRKLPPTNAG RRQKHRPACP SCDSYEKAPP KEFLERLKSL IQKMIHQHLS
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 2 ng/ml.
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Interleukin 21 (IL21) (AA 32-162) (Active) protein
IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 21 (IL21) (AA 30-162) protein (Fc Tag)IL21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Interleukin 21 (IL21) (AA 30-162) (Active) proteinIL21 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Interleukin 21 (IL21) (AA 32-162) (Active) protein (Biotin,His-Avi Tag)IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 21 (IL21) (AA 25-155) protein (His-IF2DI Tag)IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 21 (IL21) (AA 18-146) protein (His-IF2DI Tag)IL21 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 21 (IL21) protein (His tag)IL21 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 21 (IL21) (AA 1-162) protein (GST tag)IL21 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 21 (IL21) (AA 18-146) (Active) protein (Biotin,His-Avi Tag)IL21 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 21 (IL21) (AA 30-162) protein (His tag)IL21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Phosphate-Buffered Saline ( pH 7.4)
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- IL-21 (IL21) (Interleukin 21 (IL21))
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别名
- IL-21
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背景
- IL-21, also known as Interleukin-21, is an important modulator of natural killer (NK) cell function. However, little is known about IL-21 function in canine NK cells because the phenotype of these cells remains undefined. NK cells proliferated rapidly in response to activation by IL-21 for 3 weeks, and IL-21 was able to induce changes in the mRNA expression of NK cell-related receptors and enhance the effector function of NK cells in perforin- and granzyme-B-dependent manners. The duration, frequency and timing of IL-21 stimulation during culture affected the rate of proliferation, patterns of receptor expression, cytokine production, and anti-tumor activity. It has a potential predicating that synergistic interactions of IL-21 with IL-2 and IL-15 play an important role in the proliferation, receptor expression, and effector function of canine NK cells. Recombinant canine IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 15 kDa (130aa), confirmed by MALDI-TOF
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NCBI登录号
- NP_001003347
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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