NGF 2.5S (Active) protein (Atto 647N)
This Native NGF 2.5S protein is expressed in 小鼠.
产品编号 ABIN7582347
发货至:
中国
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Contact Info
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中国
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Quick Overview for NGF 2.5S (Active) protein (Atto 647N) (ABIN7582347)
抗原
NGF 2.5S
蛋白类型
Native
生物活性
Active
宿主
小鼠
资源
小鼠
应用范围
Cell Culture (CC), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI), Binding Studies (Bind), Flow Cytometry (FACS)
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标记
- Atto 647N
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原理
- mouse NGF 2.5S-ATTO Fluor-647N
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特异性
- Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors
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产品特性
- ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is one molecule of dye per one mouse NGF 2.5S molecule, linked via primary amines.
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Biological Activity Comment
- The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins.
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NGF 2.5S (Active) protein (Biotin)
宿主: 小鼠 宿主: 小鼠 Native CC, CyTox, FM, IF, LCI Active
NGF 2.5S (Active) protein宿主: 小鼠 宿主: 小鼠 Native >95 % (HPLC) CC, CyTox, ELISA Active
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应用备注
- Antigen Preadsorption Control: 4-100 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
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- NGF 2.5S
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背景
- A fluorescent mouse NGF 2.5S for sensitive detection, live-cell imaging, and tracking of TrkA and p75NTR receptors and NGF internalization dynamics
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分子量
- ~26.6 kDa (dimer)
抗原
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