产品编号 ABIN7887294
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for NGF 2.5S (Active) 蛋白 (ABIN7887294)
抗原
NGF 2.5S
蛋白类型
Native
生物活性
Active
宿主
小鼠
资源
小鼠
应用范围
Cell Culture (CC), Cytotoxicity Test (CyTox), ELISA
纯度
>95 % (HPLC)
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原理
- Native mouse NGF 2.5S protein (>95%)
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特异性
- Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors
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Biological Activity Comment
- NGF is purified in three forms: the 7S, 2.5S and β. The 2.5S form is 9 amino acids shorter than the β form because of proteolysis that occurs during the purification process1. NGF has been shown to regulate neuronal survival, development, function and plasticity2. The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins3.
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NGF 2.5S (Active) protein (Atto 647N)
宿主: 小鼠 宿主: 小鼠 Native CC, CyTox, FM, IF, LCI, Bind, FACS Active
NGF 2.5S (Active) protein (Biotin)宿主: 小鼠 宿主: 小鼠 Native CC, CyTox, FM, IF, LCI Active
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应用备注
- Antigen Preadsorption Control: EC50 = 0.7 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual acetate.
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- NGF 2.5S
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背景
- A native mouse neurotrophin purified from submaxillary glands that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
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分子量
- 26 kDa
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CAS-编号
- 93928-24-6
抗原
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