Pro BDNF Protein (Biotin)
proBDNF
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
WB, LCI
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- 抗原 See all Pro BDNF (proBDNF) products
- Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Pro BDNF protein is labelled with Biotin.
- 应用范围
- Western Blotting (WB), Live Cell Imaging (LCI)
- 原理
- Biotin-Labeled Human ProBrain-Derived Neurotrophic Factor, Recombinant, E. coli
- 序列
- MAPMKEANIR GQGGLAYPGV RTHGTLESVN GPKAGSRGLT SLADTFEHVI EELLDEDQKV RPNEENNKDA DLYTSRVMLS SQVPLEPPLL FLLEEYKNYL DAANMSMRVR RHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR (the prodomain is shown in bold)
- 特异性
- Toxin target: p75NTR, Sortilin receptors, LC-Biotin
- 产品特性
- Biotin-Labeled Human ProBrain-Derived Neurotrophic Factor, Recombinant, E. coli
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Pro-Brain-Derived Neurotrophic Factor (proBDNF) protein
proBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
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- 应用备注
- Antigen Preadsorption Control: 0.1 - 10 nM
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
- 缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
- 储存条件
- -20 °C
- 储存方法
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day.,Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.
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- 抗原
- Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))
- 别名
- proBDNF (proBDNF 产品)
- 背景
- ProBrain-Derived Neurotrophic Factor,BDNF is a neurotrophic factor produced by proteolytic cleavage of its precursor, proBDNF. The biologically relevant form of the protein was thought to be the mature form, BDNF.1 The actions of BDNF are mediated via the binding to TrkB or p75.2,3The precursor form was thought to be important for the correct folding, secretion and trafficking of the mature protein. A single-nucleotide polymorphism (Val66 to Met) in the pro-domain of the human BDNF gene impairs intracellular trafficking and regulated secretion of BDNF in primary cortical neurons and neurosecretory cells but not in endothelial and vascular cells.4 This has been shown to affect memory and lead to abnormal hippocampal function in humans.5The finding that proBDNF and not mature BDNF is the preferred ligand for p75, has ushered in a new era which reexamines the biological roles of the two forms.6 Some biological roles for proBDNF have been proposed. It has been shown to be a pro-apoptotic ligand for sympathetic neurons7 expressing both p75 and sortlin, and to be involved in LTD8. On the other hand, it has also been shown to elicit prototypical TrkB responses in biological assays, such as TrkB tyrosine phosphorylation, and activation of ERK1/2.9In brain homogenates a mixture of both, proBDNF and mature BDNF has been found10,11 and in cortical neurons secretion of proBDNF has been shown.7 Binding of both proBDNF and mature BDNF to TrkB has been proposed to be via the R103 residue in the mature portion.9
- 分子量
- ~52.5 kDa (dimer)
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