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Flagellin Protein (FliC) (AA 1-495, active-Mutant)

This Recombinant Flagellin protein is expressed in 大肠杆菌（E. Coli）.

产品编号 ABIN7566155

发货至: 中国

Quick Overview for Flagellin Protein (FliC) (AA 1-495, active-Mutant) (ABIN7566155)

抗原

See all Flagellin (FliC) 蛋白

Flagellin (FliC)

蛋白类型

Recombinant

生物活性

Active

宿主

3
2
2
2
2
2
1
1
1

Salmonella typhimurium

资源

大肠杆菌（E. Coli）

纯度

>95 % (SDS-PAGE)

Discover our top product FliC 蛋白

产品特性

AA 1-495, active-Mutant

原理

Flagellin (rec.) (untagged) (highly active)

交叉反应

人, 小鼠

产品特性

Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495).

内毒素水平

<0.001EU/μg purified protein (LAL test, Lonza).

Biological Activity Comment

Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse.
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Flagellin (FliC) (AA 2-498) protein (His tag)
FliC 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB

Flagellin (FliC) protein
FliC 宿主: Salmonella typhimurium 宿主: Salmonella typhimurium Native high purity

Flagellin (FliC) (Mutant) protein (His tag)
FliC 宿主: 人, 小鼠宿主: 大肠杆菌（E. Coli） Recombinant >95 % (SDS-PAGE)

Flagellin (FliC) protein
FliC 宿主: Salmonella typhimurium 宿主: Salmonella typhimurium Native high purity

Flagellin (FliC) (AA 1-495) (Active) protein (His tag)
FliC 宿主: 人, 小鼠宿主: 大肠杆菌（E. Coli） Recombinant >95 % (SDS-PAGE) SDS Active

Flagellin (FliC) protein
FliC 宿主: Borrelia garinii 宿主: 大肠杆菌（E. Coli） Recombinant WB, LF, EIA

Flagellin (FliC) protein
FliC 宿主: Borrelia burgdorferi 宿主: 大肠杆菌（E. Coli） Recombinant ELISA, WB, SDS, CP

Flagellin (FliC) (AA 1-160) protein (His tag)
FliC 宿主: Bacillus subtilis 宿主: 酵母菌 Recombinant > 90 % ELISA

Flagellin (FliC) (AA 13-216) protein (His tag)
FliC 宿主: Methanococcus 宿主: 酵母菌 Recombinant > 90 % ELISA

Flagellin (FliC) (AA 13-222) protein (His tag)
FliC 宿主: Methanococcus 宿主: 酵母菌 Recombinant > 90 % ELISA

限制

仅限研究用
状态

Lyophilized

溶解方式

1 mg/mL after reconstitution.

浓度

1 mg/mL

缓冲液

Contains PBS.

注意事项

After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

储存条件

4 °C,-20 °C

储存方法

Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
抗原

Flagellin (FliC)

别名

Flagellin

背景

FliC
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

分子量

~54kDa (SDS-PAGE)

UniProt

P06179

途径

Inflammasome

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