CHAF1A
(Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))
蛋白类型
Recombinant
产品特性
AA 1-911
宿主
小鼠
资源
HEK-293 Cells
标记
This CHAF1A protein is labelled with His tag.
原理
Made-to-order recombinant Chaf1a Protein expressed in mammalian cells.
序列
MLEEPEAATR TAAAVDCKDR PGFPVKRLIQ ARLPFKRLNL VPKEKVEEDT SPKAAVESKV PDLQLSLGTF ESQCHTGSHV GLSTKLVGGQ GPIDSFLRAT IKPVPSVVII DLTENCSDIP DSPEGHSELS PDTAGVVTTV EGAAKQQEHS AAELCLLETP SDITCHMEEE PGSPGDPKRT GDCQAGSLQS CPELTPGSRT CPTKELSSWS KAGDLLFIEK VPVVVLEDIL ATKPSIASLP MMSLDRSVTS ESEILESCPE DDSILSHSST NSSSPTSSPE GPSTPPEHRG GRSSPSTPAC RVAKNFVKGS TEKGRSKLHR DREQQREEKE KLREEIRRAK EEARKKKEEE KELKEKERRE KREKDEKEKA EKQRLKEEKR KERQEALEAK LEEKRKKEEE KRLREEEKRL REEEKRIKAE KAEITRFFQK PKTPQAPKTL AGSCGKFAPF EIKEHMVLAP RCRAALDQDL CDQLDQLLQQ QSVASTFLSD LKSRLPLRSG PTRVCGHDTD IMNRDVVIVE SSKVDGVSER KKFGRMKLLQ FSENHRPAYW GTWNKKTAII RPRNPWAQDK DLLDYEVDSD DEWEEEEPGE SLSHSEGDED DDVGEDEDED DGFFVPHGYL SEDEGVTEEC ADPENHKVHQ KLKAKEWDEL LAKGKRFRVL QPVHVGCVWA SEAANCTSSD LKLLQQFTAC LLDVASPDEP EPGASRREKR DQHILAQLLP LLHGNVNGSK VIIHEFQEQC RRGLLTLPSP TPHLQMPNLE DAVAVPSKAR LKRLISENSA YEKRPNFRMC WYVHPEVLKS FGQECLPVPC QWTYITTMPS APREDSGSAS TEGPGQSTPM LLKRKPAATM CITQFMKKRR YDGQVGSGDM DGFQADTEED EEDDTDCMII DVPDVGSDVS EAPIPAPTLC K Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
CHAF1A
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
custom-made
CHAF1A
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
CHAF1A
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
CHAF1A
(Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))
Chromatin assembly factor 1 subunit A (CAF-1 subunit A) (Chromatin assembly factor I p150 subunit) (CAF-I 150 kDa subunit) (CAF-I p150),FUNCTION: Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. {ECO:0000250|UniProtKB:Q5R1T0}.