USP14 Protein (AA 1-493) (His tag)
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- 抗原 See all USP14 蛋白
- USP14 (Ubiquitin Specific Peptidase 14 (USP14))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-493
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This USP14 protein is labelled with His tag.
- 原理
- Predefined custom protein recombinant Usp14 Protein expressed in mammalian cells.
- 序列
- MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG GTLKDDDWGN IKMKNGMTVL MMGSADALPE EPSAKTVFVE DMTEEQLATA MELPCGLTNL GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ YITAALRDLF DSMDKTSSSI PPIILLQFLH MAFPQFAEKG EQGQYLQQDA NECWIQMMRV LQQKLEAIED DSGRETDSSS APAVTPSKKK SLIDQYFGVE FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL KLRLQEEITK QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP LMLDVYELCT PELQEKMVSF RSKFKDLEDK KVNQQPNAND KNSPPKEIKY EPFSFADDIG SNNCGYYDLQ AVLTHQGRSS SSGHYVSWVR RKQDEWIKFD DDKVSIVTPE DILRLSGGGD WHIAYVLLYG PRRVEIMEEE SEQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
- Top Product
- Discover our top product USP14 蛋白
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Ubiquitin Specific Peptidase 14 (USP14) (AA 1-494) protein (His tag)
custom-made USP14 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin Specific Peptidase 14 (USP14) (AA 1-494) protein (GST tag)USP14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ubiquitin Specific Peptidase 14 (USP14) proteinUSP14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Ubiquitin Specific Peptidase 14 (USP14) (Transcript Variant 2) protein (His tag)USP14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin Specific Peptidase 14 (USP14) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)USP14 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- USP14 (Ubiquitin Specific Peptidase 14 (USP14))
- 别名
- Usp14 (USP14 产品)
- 背景
- Ubiquitin carboxyl-terminal hydrolase 14 (EC 3.4.19.12) (Deubiquitinating enzyme 14) (Ubiquitin thioesterase 14) (Ubiquitin-specific-processing protease 14),FUNCTION: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed:16190881). Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (By similarity). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (PubMed:19726649). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (By similarity). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (By similarity). {ECO:0000250|UniProtKB:P54578, ECO:0000269|PubMed:16190881, ECO:0000269|PubMed:19726649}.
- 分子量
- 56.0 kDa
- UniProt
- Q9JMA1
- AlphaFold
- Q9JMA1
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