USP14 Protein (AA 91-494, C-Term) (His tag)
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- 抗原 See all USP14 蛋白
- USP14 (Ubiquitin Specific Peptidase 14 (USP14))
- 蛋白类型
- Recombinant
- 产品特性
- AA 91-494, C-Term
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This USP14 protein is labelled with His tag.
- 原理
- Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase 14/USP14 (N-6His)
- 序列
- MGSSHHHHHH SSGLVPRGSH MDMTEEQLAS AMELPCGLTN LGNTCYMNAT VQCIRSVPEL KDALKRYAGA LRASGEMASA QYITAALRDL FDSMDKTSSS IPPIILLQFL HMAFPQFAEK GEQGQYLQQD ANECWIQMMR VLQQKLEAIE DDSVKETDSS SASAATPSKK KSLIDQFFGV EFETTMKCTE SEEEEVTKGK ENQLQLSCFI NQEVKYLFTG LKLRLQEEIT KQSPTLQRNA LYIKSSKISR LPAYLTIQMV RFFYKEKESV NAKVLKDVKF PLMLDMYELC TPELQEKMVS FRSKFKDLED KKVNQQPNTS DKKSSPQKEV KYEPFSFADD IGSNNCGYYD LQAVLTHQGR SSSSGHYVSW VKRKQDEWIK FDDDKVSIVT PEDILRLSGG GDWHIAYVLL YGPRRVEIME EESEQ
- 产品特性
- Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase 14/USP14 is produced by our E. coli expression system. The target protein is expressed with sequence (Asp91-Gln494) of Human USP14.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product USP14 蛋白
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Ubiquitin Specific Peptidase 14 (USP14) (AA 1-494) protein (GST tag)
USP14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ubiquitin Specific Peptidase 14 (USP14) (AA 395-494) protein (GST tag)USP14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ubiquitin Specific Peptidase 14 (USP14) (AA 1-494) protein (Strep Tag)Crystallography grade USP14 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ubiquitin Specific Peptidase 14 (USP14) (AA 1-493) protein (Strep Tag)Crystallography grade USP14 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ubiquitin Specific Peptidase 14 (USP14) (Transcript Variant 2) protein (His tag)USP14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin Specific Peptidase 14 (USP14) (AA 1-494), (full length) protein (His tag)USP14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Ubiquitin Specific Peptidase 14 (USP14) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)USP14 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 20 % Glycerol, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- USP14 (Ubiquitin Specific Peptidase 14 (USP14))
- 别名
- usp14 (USP14 产品)
- 别名
- TGT Protein, DDBDRAFT_0202821 Protein, DDBDRAFT_0237695 Protein, DDB_0202821 Protein, DDB_0237695 Protein, 2610005K12Rik Protein, 2610037B11Rik Protein, AW107924 Protein, C78769 Protein, ax Protein, zgc:55949 Protein, wu:fk63d09 Protein, MGC81945 Protein, tgt Protein, MGC69343 Protein, USP14 Protein, ubiquitin specific peptidase 14 Protein, peptidase C19 family protein Protein, ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) Protein, ubiquitin specific protease 14 Protein, ubiquitin specific peptidase 14 S homeolog Protein, universal stress protein Protein, Ubiquitin carboxyl-terminal hydrolase 14 Protein, USP14 Protein, usp14 Protein, Usp14 Protein, Bm1_28765 Protein, usp14.S Protein, RR_RS06480 Protein, usp-14 Protein
- 背景
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Ubiquitin Carboxyl-Terminal Hydrolase 14 (USP14) belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 located in the cytoplasm is a proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. In addition, USP14 is indispensable for synaptic development and function at neuromuscular junctions, required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.
Alternative Names: Ubiquitin Carboxyl-Terminal Hydrolase 14, Deubiquitinating Enzyme 14, Ubiquitin Thioesterase 14, Ubiquitin-Specific-Processing Protease 14, USP14, TGT - 分子量
- 48.45 kDa
- UniProt
- P54578
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