CC2D1A
(Coiled-Coil and C2 Domain Containing 1A (CC2D1A))
蛋白类型
Recombinant
产品特性
AA 1-943
宿主
小鼠
资源
HEK-293 Cells
标记
This CC2D1A protein is labelled with His tag.
原理
Made-to-order recombinant Cc2d1a Protein expressed in mammalian cells.
序列
MHKRNGPQAP PGRGAVTARQ LGLLVDFSPD GLMIPEDGIN EEELEAEFLA LVGGQPQALE KLKGQGPLPM EAIEKMARLC MRDLDEDEEG TDEDDVEADE DLLAELNEVL GEEQKAVEPL MPVAQPKPSG PNPGVEATLQ ERLTLYQSAL ESARQAGDSA KMRRYDRGLK TLENLLVSAK KGNIINEADI PPPVASGKGA AAGHSHTQAT SQLASVSPPA PESSGTLEAP STTTPTSAKP QLPPDPCSPL ARLQSLQHEY KLAALRAKHQ DDTATATRHL RIAKSFDPVL EALSRGELVD LSRLPPPPDQ LSPEPPLPAA QPLTSASTLT RPEVPQPPRN LLEALEQRME RYHVAAAQAK AKGDQRKARM HERIVKQYQD AIRAHKAGRA VDVAELPVPP GFPPMQGLES AEPSQQSLVG VLETAMRLAN HDEGSDDEEE ETPKKQNTPA ASTTQLKSSP SKAPPSGPAP AGKAAPKGTS NRAQQQLAFL EGRKKQLLQA ALRAKQKNDV EGAKMHLRQA KGLEPMLEAS RNGLPVDIAK VPPAPVNKDD FVLVQRPGPG LSQEAVRRYG ELTKLLRQQH EMCLNHSTQF THLGNIAETI KFEKLAEDCK RSMDTLKQAF ARSLPTPAAR FEQRTFSVIK VFPDLSNSDM LLFIVKGINL PTPTGLSPSD LDAFVRFDFP YPNVEEAQKD KTSVIKNTDS PEFKEQFKLC INRGHRGFRR AIQTKGIKFE VVHKGGLFKT DRVLGTAQLK LGTLETACEV HEILEVLDGR RPTGGRLEVM VRIREPLTAQ QLETTTERWL VIDHIPAAMP TVTGPKAKAP LIPASSREAG NRSARPLHSL SVLAFDQERL ERKILALRQA RRPVPPEVAQ QYQDVVQRSQ WQRAQLEQGG AALRREYASH LERQLHFYTE AARRLGYDGS REAAKEALYR RNLVESELQR LRR Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
CC2D1A
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
WB, SDS, ELISA
custom-made
CC2D1A
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
WB, SDS, ELISA
CC2D1A
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
CC2D1A
(Coiled-Coil and C2 Domain Containing 1A (CC2D1A))
Coiled-coil and C2 domain-containing protein 1A (Five prime repressor element under dual repression-binding protein 1) (FRE under dual repression-binding protein 1) (Freud-1),FUNCTION: Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. May play a role in the altered regulation of HTR1A associated with anxiety and major depression. Mediates HDAC-independent repression of HTR1A promoter in neuronal cell. Performs essential function in controlling functional maturation of synapses. {ECO:0000269|PubMed:12917378, ECO:0000269|PubMed:14756806, ECO:0000269|PubMed:21273312}.