Made-to-order recombinant Oplah Protein expressed in mammalian cells.
序列
MGSPEERFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG IRRILEQERG VLLPRGRPLD TSHIASIRMG TTVATNALLE RQGERVALLV TRGFRDLLHI GTQARPDLFD LAVPMPEVLY EEVVEVDERV LLYRGEPGAG SPVKGCTGDL LEIQQPVDLA ALRGKLEGLL TRGIHSLAVV LMHSYTWAQH EQQVGTLARE LGFTHVSLSS EVMPMVRIVP RGHTACADAY LTPTIQRYVQ GFRRGFQGQL KNVQVLFMRS DGGLAPMDAF SGSRAVLSGP AGGVVGYSTT TYQLEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR LLPASFPCIF GPGEDQPLSP EASRKALEAV AMEVNSFLAS GPCPASQLSL EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLSYT PETFAQLDQR LSRLEEQCVD ALQAQGFSRS QISTESFLHL RYQGTDCALM VSANQHPATT CSPRAGDFGA AFVERYMREF GFIIPERSVV VDDVRVRGTG RSGLQLEETS KIQSGPPHVE KVTQCYFEGG YQETPVYLLG ELGYGHQLQG PCLIIDNNST ILVEPGCQAE VIETGDIRIS VGAEAPSMID TKLDPIQLSI FSHRFMSIAE QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNAPHIP VHLGAMQETV QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQSRPVFYV ASRGHHADIG GITPGSMPPH STTLQQEGAV FLSFKLVQGG VFQEEAVTEA LQAPGKISGC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ AYMGHIQANA ELAVRDMLRA FGTSRQARGL PLEVSAKDHM DDGSPICLHV QINLNQGSAV FDFSGSGSEV FGNLNAPRAI TLSALIYCLR CLVGRDIPLN QGCLAPVQVI IPKGSILDPS PEAAVVGGNV LTSQRVVDVI LGAFGACAAS QGCMNNVTLG NARMGYYETV AGGAGAGPGW HGRSGVHSHM TNTRITDPEI LESRYPVILR RFELRPGSGG RGRFRGGDGV VRELVFREEA LLSVLTERRA FQPYGLHGGE PGTRGLNLLI RKDGRTVNLG GKTSVTVYPG DAFCLHTPGG GGYGDPEDPA PPPGSPPLYP AFPERGSVYE YRRAQEAV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
OPLAH
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
custom-made
OPLAH
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
OPLAH
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
5-oxoprolinase (EC 3.5.2.9) (5-oxo-L-prolinase) (5-OPase) (Pyroglutamase),FUNCTION: Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. {ECO:0000250|UniProtKB:Q75WB5}.