Made-to-order recombinant Depdc1a Protein expressed in mammalian cells.
序列
MERQGAPLGP YRATKLWNEV TTSFRVGMPL RKHRQHLKKY SNCFTAVEAI DWLYDLLRSN SNFGPEVTRQ QTIQLLRKFL KNHVIEDIKG RWGSESLDDN NQLFRFPATS PLKTFPQRHT EIKKTNTEYF SKDKDGIFKL RNLSQKTSKK RGLHFSQENT EKINHERITN EDLEIAPDNQ EISQEDVEEV WRYVIMIYLQ TILSLPSIEE LLNPNQVIPQ YIMYNMANTS KHGVVILQDK SDDLPHWVLS AMKCLANWPR SNDTNNLTYV GFERDVFKTI ADYFLNLPEP LLTFEYYELF VNILVVCGYI TVSDRTSGIH KIQDDPRSSK IHDLSNLNSF KSTECLLLSL LYKDKSNEEL DSTKRLQRND QGFQERCAKK MQLDNLRNRR ASANDIMGGS CHNLIGLSNT NALSSNIKPR CSSLEGIVDR PVNSSEKKSS IFYQSVLNIE EQNSKQSLVS APKQTPLFNL HSDENAQQPH CVGFNRTSAL TVQDQEELCN EKYKSKQLCR SQSLLLRSST RQNSCINKPV AEIIMKPNVG QGSTSELGES STTINKRLCK STIELSEKSL PPAASVLTGT QSLLQPHLER VAINALQLCC LLLPPPNRRK LQLLMRMISR MSQNVDMPKL HEQIGTRSLM INTFSRCVLC CAEEVDLDEL LASRLVSFLM DHHQEILQVP TYLQAAVEKH LDYIKKGNVK NHGDGLVVPL PTYSYCKQIS AKEFDEQKIS TSQAAIAELL ENIVRSKSLS LKEKRRKLKQ KEYPLIYQKR FPTTESEAAL FDDKPTIKQP MLNLRNPKLH SLRY Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
DEPDC1
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
custom-made
DEPDC1
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
DEP domain-containing protein 1A,FUNCTION: May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells (By similarity). {ECO:0000250}.