> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
质量等级
custom-made
产品特性
AA 1-905
标记
This ZFYVE28 protein is labelled with His tag.
原理
Predefined custom protein recombinant Zfyve28 Protein expressed in mammalian cells.
序列
MMNRFRKWLY KPKRSDPQLL AQFYYADEEL NQVAAELDSL DGRKEPQRCT LLVSQFRSCQ DNVLNIINQI MEECIPQDRA PRDFCVKFPE EIRHDNLAGQ LWFGAECLAA GSIIMNRELE SMAMRPLAKE LTRSLEDVRG TLRDQALRDL NTYTEKMREA LRRFDVLFAE FELSYVSAMV PVKSPREYYV QQEVIVLFCE TVERALDFGY LTQDMIDDYE PALMFTIPRL AIVCGLVVYA DGPLNLDRKV EDMSELFRPF HTLLRKIRDL LQALTEEELH TLERSLCVSQ DVELPIRADT QAPSALAPTF SASLPPEETL SASANNPEAE LACSMQYDDQ ELEELSRMVH RAGDEMSSLL SPPSACQSPA HRPGSEASPR GEASPARARL KSGSDEEERV FFMDDVEVTE SPARPESPGN TFELTQGNAQ QRGQDGQSGE VGVEAPALVK EEDWSNNNVE GDKIKLASLM GSTSCSCLDS QLYLDGWEVS AEDAETAEMI AHRTGGMKLS ATVIFNPKSP TSQDSAVAAQ EAPGHGTSPL EPRAEGTGDN SHKLSTTATN CLLHSCVCCG SCGDSRDDAV ERLREKCGPG SVISASNPSV SLAKGGDKEP ERIDEAQPSD VTLPAEDASN RQEPKAPASS KCLAHTSGPQ VDTASRLQGE GEVKGQPEPE ARKQDPEKSP VVSGDSPRGD VAQTEHQHLL GSSSTVGSCS LDNTRLDVAT AAMPVTPMAT REKIRSRFHG SHDLIHRLFV CISGVADQLQ TNYASDLRSI LKTLFEVMAT KPETDDKEKL KKVTQTLRSA ALEDCALCQE TVSSSELAAK TRDGDFEDPP EWVPDEACGF CTSCKAPFTV IRRKHHCRSC GKIFCSRCSS HSAPLPRYGQ VKPVRVCTHC YMFHVTPFYS DKTGM Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Predefined custom protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
custom-made
ZFYVE28
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
ZFYVE28
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
Lateral signaling target protein 2 homolog (Zinc finger FYVE domain-containing protein 28),FUNCTION: Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated (By similarity). {ECO:0000250}.