Made-to-order recombinant Kif15 Protein expressed in mammalian cells.
序列
MAPGCKSELR NVTNSHSNQP SNEGDAIKVF VRIRPAEEGA RSADGEQSFC LSVLSQTTLR LHSNPDPKTF VFDYVAGMDT TQESVFSTVA KSIVESCMSG YNGTIFAYGQ TGSGKTFTMM GPSDSDNFSH NLRGIIPRSF EYLFSLIDRE KEKAGAGKSF LCKCSFIEVY NEQIYDLLDS ASVGLYLREH IKKGVFVVGA VEQAVTSAAE TYQVLSRGWR NRRVASTSMN RESSRSHAVF TITIESMEKS SETVNIRTSL LNLVDLAGSE RQKDTHAEGM RLKEAGNINR SLSCLGQVIT ALVDVGNGKQ RHICYRDSKL TFLLRDSLGG NAKTAIIANV HPGSRCFGET LSTLNFAQRA KLIKNKAVVN EDTQGNVSQL QAEVKRLKEQ LSQFTSGQIT PESLLARDKE KTNYIEYFLE AMLFFKKSEQ EKKSLIEKIT QLEDLTLKKE KFIQSNKMIV KFREDQIMRL ERLHKEGRGS FLPEEQDRLL SELRDEVQTL REHVEHHPRL AKYAMENHSL REENRRLKLL APVKRAHEID AQSIARLEKA FAEVSSTETN DKGLQGFSPK ALKESSFFTN TEKLKAQLLQ IQTELNNSKQ EYEEFKELTR KKQLELESEL QSLQKANLNL ENLLEATKVC KRQEVSQLNK LHAETLKIIT TPTKAYQLCS RLVPKSSPEV GSFGFLCTES SSRLDNDILN EPVPPEMSEQ ALEAVSEELR TVQEQLSVLQ VKLDEEERKN LKLQQNVDKL EHHSTQMQEL FSSERSDWTK QQQEHVTQLS DLEKQLQDAQ TKNEFLKCEV HDLRIVLNSA DKELSLVKLE YSTFKENHEK ELSQLSERHV QVQLQLDNAR LENEKLLESQ ACLQDSYDNL QEVMKFEIDQ LSKNLQNCKQ ENETLKSDLH NLVELFEAEK ERNNKLSLQF EEDKENSSKE ILKVLETVRQ EKQKEMAKCE KQMAKIQKLE ESLLATENVI SSLEKSRESD KELVTNLMNQ IQELRISIGE KSETIATLKQ ELQDINCKYN ASLADKEESK ELIRRQEVDI LELKETLRLR ILSEDIERDM LCEDLAHATE QLNMLTEASK KHSGLLQSAQ EELTRKEALI QELQHKLNQE KEEVEQKKNE FSLKMRQLEH VMGSATEYPQ SPKTPPHFQA HLAKLLETQE QEIEDGRASK TSLQHLVTKL NEDREVKNAE ILRMKDQLCE MENLRLESQQ LREKNWLLQR QLDDVKRQQE SGDQSHPDSQ QLKNEHEEII KERLAKNKLI EEMLKMKTNL EEVQSALHSK EKACHRMSEE IERTRTLESR AFQEKEQLRS KLEEMYEERE RTFLEMEMLK KQLEFLAEEN GKLVGHQNLH QKIQYVVRLK KENIRLTEET EKLRAENLFL KEKKKEF Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
KIF15
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
KIF15
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
Kinesin-like protein KIF15 (Kinesin-like protein 2) (Kinesin-like protein 7),FUNCTION: Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly. {ECO:0000250}.