Predefined custom protein recombinant Arap1 Protein expressed in mammalian cells.
序列
MAEGYDAALS VAEWLRALHL EQYTALFEQH GLVWATECQG LSDAGLLDMG MHLPGHRRRI LAGLHRAHAP PVPLPRPAPR PVPMKRHIFR SPPVPVTPPE PPPTAGEDEG LPAAPPIPPR RSCLPPACFT PTSTAAPDPV LPPLPAKRHL VEPSVPPVPP RTGPPYPQAS LLAKEELLLP SVSPRSQPEP AETPSTLLPA FPQGPLQPPS PPPCPPVIPP KPPRLLPEFD DSDYDDVPEE GPGAPASVMT KEEPLPSRVP RAVRVASLLS EGEELSGDDS EDDDDHAYEG IPNGGWPTSG LNPPLRSLIP DLPLHPMDEL PGGPTPITPV IKAGWLDKNP PQGSYIYQKR WVRLDADYLR YFDSNKDAYS KRFVPVACIC RVAPIGDQKF EVITNNRTFA FRAESDVERN EWMQALQQAV VEHRARFRLS SASVLGVRGS EQPDRAGSLE LRGFKNKLYV AVTGDKVQLY KNLEEFHLGI GITFIDMNVG NVKEVDRRSF DLTTPYRIFS FSADSELEKE QWLEAMQGAI AEALSTSEVA ERIWAAAPNR FCADCGAAQP DWASINLCVV ICKRCAGEHR GLGAGVSKVR SLKMDRKVWT EALIQLFLHL GNGPGNHFWA ANVPPSEALE PSSSPGARRY HLEAKYREGK YRRYHPLFGN QEELDKALCA AVTTTDLAET QALLGCGAGV SCFSGDPAAP TPLALAEQAG QTLQMEFLRN NQSTEVPRLD SVKPLEKHYS VTLPTVSHSG FLYKTASAGK PLQDRRAREE FSRRWCVLSD GVLSYYENER AVTPNGEIRA SEIVCLAVSP LDTHGFEHTF EVYTEGERLY LFGLENAELA HEWVKCIAKA FVPPLAEDLL ARDFERLGRL PCKAGLSLQQ AQEGWFALTG SELRAVFPEG PWEEPLQLRK LQELSIQGDS ENQVLVLVER RRTLYIQGER RLDFMAWLGV IQKAAASLGD TLSEQQLGDS DIPVIVYRCV DYITQCGLTS EGIYRKCGQT SKTQRLLDSL RQDARSVHLK EGEQHVDDVS SALKRFLRDL PDGLFTRAQR LAWLEASEIE DEEEKISRYR ELLVHLPPVN RATVKALISH LYCVQCFSDT NQMNTHNLAI VFGPTLFQTD GQDYKAGKVV EDLINHYVVV FSVDEEELRK QREEVTAIVK MRVAGTASGT QHAGDFICTV YLEEKKVETE QHVKIPASMT AEELTLEILD RRNVSIREKD YWTCFEVNEK EEAERPLHFA EKVLPIVHGL GIDSHLVVKK YQSMEAMLLY LASRVGDTKH GMMKFREDRS LLGLGLPSGG FHDRYFILNS SCLRLYKEVR SQRPWSGAPE TSHRPEKEWP VKSLKVYLGV KKKLRPPTCW GFTVVHETEK HEKQQWYLCC DTQMELREWF ATFLSVQHDG LVWPSEPSRV SRAVPEVRMG SVSLIPLRGS ENEMRRSVAA FTADPLSLLR HV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Predefined custom protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
custom-made
ARAP1
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
ARAP1
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
ARAP1
(ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1))
别名
Arap1
背景
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (Centaurin-delta-2) (Cnt-d2),FUNCTION: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity). {ECO:0000250}.