GPR31 Protein (AA 1-319) (His tag)
custom-made
GPR31
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 抗原 See all GPR31 蛋白
- GPR31 (G Protein-Coupled Receptor 31 (GPR31))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-319
- 宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This GPR31 protein is labelled with His tag.
- 原理
- Predefined custom protein recombinant Gpr31 Protein expressed in mammalian cells.
- 序列
- MERTNCSAAS TVVETAVGTM LTLECVLGLM GNAVALWTFF YRLKVWKPYA VYLFNLVVAD LLLATSLPFF AAFYLKGKTW KLGHMPCQVL LFLLAFSRGV GVAFLTTVAL DRYLRVVHPR LRVNLLSLRA AWGISSLIWL LMVVLTPQNL LTCRTTQNST ECPSFYPTGG AKAIATCQEV LFFLQVLLPF GLISFCNSGL IRTLQKRLRE SDKQPRIRRA RVLVAIVLLL FGLCFLPSVL TRVLVHIFQE FKSCSVQQAI VRASDIAGSL TCLHSTLSPA IYCFSNPAFT HSYRKVLKSL RGRRKAAESP SDNLRDSYS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (His tag)
custom-made GPR31 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (GST tag)GPR31 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- GPR31 (G Protein-Coupled Receptor 31 (GPR31))
- 别名
- Gpr31 (GPR31 产品)
- 背景
- 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor (12-(S)-HETE receptor) (12-HETER) (G-protein coupled receptor 31),FUNCTION: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE), with much lower affinities for other HETE isomers (By similarity) (PubMed:29227475). 12-S-HETE is a eicosanoid, a 12-lipoxygenase (ALOX12) metabolite of arachidonic acid, involved in many physiologic and pathologic processes, such as cell growth, adhesion, inflammation and cancer promotion. 12-S-HETE-binding leads to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B pathways and leads to cell growth. Plays a crucial role for proliferation, survival and macropinocytosis of KRAS-dependent cancer cells by mediating the translocation of KRAS from the endoplasmic reticulum to the plasma membrane (PM) and its association with the PM (By similarity). Contributes to enhanced immune responses by inducing dendrite protrusion of small intestinal CX3CR1(+) phagocytes for the uptake of luminal antigens (PubMed:30675063). Acts also as a key receptor for 12-(S)-HETE-mediated liver ischemia reperfusion injury (PubMed:29227475). {ECO:0000250|UniProtKB:O00270, ECO:0000269|PubMed:29227475, ECO:0000269|PubMed:30675063}., FUNCTION: Proton-sensing G protein-coupled receptor. {ECO:0000250|UniProtKB:O00270}.
- 分子量
- 35.6 kDa
- UniProt
- F8VQN3
- AlphaFold
- F8VQN3
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