RNF4 Protein (AA 1-194) (His tag)
Quick Overview for RNF4 Protein (AA 1-194) (His tag) (ABIN7561839)
抗原
See all RNF4 蛋白蛋白类型
宿主
资源
纯度
质量等级
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产品特性
- AA 1-194
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标记
- This RNF4 protein is labelled with His tag.
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原理
- Predefined custom protein recombinant Rnf4 Protein expressed in mammalian cells.
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序列
- MSTRNPQRKR RGGTVNSRQT QKRTRETTST PEVSLETEPI ELVETVGDEI VDLTCESLEP VVVDLTHNDS VVIVEERRRP RRNGRRLRQD HADSCVVSSD DEELSRDKDV YVTTHTPRST KDDGATGPRP SGTVSCPICM DGYSEIVQNG RLIVSTECGH VFCSQCLRDS LKNANTCPTC RKKINHKRYH PIYI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
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特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
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产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Escherichia coli (E. coli)Conjugate His tag,GST tagOrigin MousePrice 4,017.41 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin MousePrice 3,993.98 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Ring Finger Protein 4 (RNF4) (AA 1-190) protein (His tag)
custom-made RNF4 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 4 (RNF4) (AA 1-190) protein (His tag)RNF4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ring Finger Protein 4 (RNF4) (AA 1-190) protein (GST tag)RNF4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Ring Finger Protein 4 (RNF4) proteinRNF4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA, IP
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应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The buffer composition is at the discretion of the manufacturer.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- RNF4 (Ring Finger Protein 4 (RNF4))
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别名
- Rnf4
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背景
- E3 ubiquitin-protein ligase RNF4 (EC 2.3.2.27) (RING finger protein 4),FUNCTION: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed:20681948). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (By similarity). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (By similarity). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (By similarity). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (By similarity). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (By similarity). {ECO:0000250|UniProtKB:P78317, ECO:0000269|PubMed:20681948}.
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分子量
- 21.9 kDa
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UniProt
- Q9QZS2
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AlphaFold
- Q9QZS2
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
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