TPCN1 Protein (AA 1-817) (His tag)
custom-made
TPCN1
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 抗原 See all TPCN1 蛋白
- TPCN1 (Two Pore Segment Channel 1 (TPCN1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-817
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This TPCN1 protein is labelled with His tag.
- 原理
- Made-to-order recombinant Tpcn1 Protein expressed in mammalian cells.
- 序列
- MAVSLDDDVP LILTLDEAES APLPPSNSLG QEQLPSKNGG SHSIHNSQVP SLVSGADSPP SSPTGHNWEM NYQEAAIYLQ EGQNNDKFFT HPKDARALAA YLFVHNHFFY MMELLTALLL LLLSLCESPA VPVLKLHTYV HATLELFALM VVVFELCMKL RWLGFHTFVR HKRTMVKTSV LVVQFIEAIV VLVRQTSHVR VTRALRCIFL VDCRYCGGVR RNLRQIFQSL PPFMDILLLL LFFMIIFAIL GFYLFSTNPS DPYFSTLENS IVNLFVLLTT ANFPDVMMPS YSRNPWSCVF FIVYLSIELY FIMNLLLAVV FDTFNDIEKH KFKSLLLHKR TAIQHAYGLL ASQRRPAGIS YRQFEGLMRF YKPRMSARER FLTFKALNQS NTPLLSLKDF YDIYEVAALQ WKAKRNRQHW FDELPRTAFL IFKGINILVN SKAFQYFMYL VVAVNGVWIL VETFMLKGGN FTSKHVPWSY LVFLTIYGVE LFMKVAGLGP VEYLSSGWNL FDFSVTAFAF LGLLALTLNM EPFYFIVVLR PLQLLRLFKL KKRYRNVLDT MFELLPRMAS LGLTLLTFYY SFAIVGMEFF NGRLTPNCCN TSTVADAYRF INHTVGNKTK VEEGYYYLNN FDNILNSFVT LFELTVVNNW YIIMEGVTSQ TSHWSRLYFM TFYIVTMVVM TIIVAFILEA FVFRMNYSRK SQDSEVDSGI VIEKEMSKEE LMAVLELYRE ERGTSSDVTR LLDTLSQMEK YQQNSMVFLG RRSRTKSDLS LKMYQEEIQE WYEEHAREQE QQKLRGSVPG PAAQQPPGSR QRSQTVT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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Two Pore Segment Channel 1 (TPCN1) (AA 1-816) protein (His tag)
custom-made TPCN1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- TPCN1 (Two Pore Segment Channel 1 (TPCN1))
- 别名
- Tpcn1 (TPCN1 产品)
- 背景
- Two pore calcium channel protein 1 (Voltage-dependent calcium channel protein TPC1),FUNCTION: Intracellular channel initially characterized as a non-selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a voltage-gated highly-selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate) that senses pH changes and confers electrical excitability to organelles (PubMed:24776928, PubMed:23394946, PubMed:23063126). Localizes to the early and recycling endosomes membranes where it plays a role in the uptake and processing of proteins and regulates organellar membrane excitability, membrane trafficking and pH homeostasis (PubMed:28855648). Ion selectivity is not fixed but rather agonist-dependent and under defined ionic conditions, can be readily activated by both NAADP and PI(3,5)P2 (By similarity). Required for mTOR-dependent nutrient sensing (PubMed:23394946). {ECO:0000250|UniProtKB:Q8NHX9, ECO:0000269|PubMed:23063126, ECO:0000269|PubMed:23394946, ECO:0000269|PubMed:24776928, ECO:0000269|PubMed:28855648}.
- 分子量
- 94.5 kDa
- UniProt
- Q9EQJ0
- AlphaFold
- Q9EQJ0
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