CYP2S1
(Cytochrome P450, Family 2, Subfamily S, Polypeptide 1 (CYP2S1))
蛋白类型
Recombinant
产品特性
AA 1-501
宿主
小鼠
资源
HEK-293 Cells
标记
This CYP2S1 protein is labelled with His tag.
原理
Made-to-order recombinant Cyp2s1 Protein expressed in mammalian cells.
序列
MEAASTWALL LALLLLLLLL SLTLFRTPAR GYLPPGPTPL PLLGNLLQLR PGALYSGLLR LSKKYGPVFT VYLGPWRRVV VLVGHDAVRE ALGGQAEEFS GRGTLATLDK TFDGHGVFFA NGERWKQLRK FTLLALRDLG MGKREGEELI QAEVQSLVEA FQKTEGRPFN PSMLLAQATS NVVCSLVFGI RLPYDDKEFQ AVIQAASGTL LGISSPWGQA YEMFSWLLQP LPGPHTQLQH HLGTLAAFTI QQVQKHQGRF QTSGPARDVV DAFLLKMAQE KQDPGTEFTE KNLLMTVTYL LFAGTMTIGA TIRYALLLLL RYPQVQQRVR EELIQELGPG RAPSLSDRVR LPYTDAVLHE AQRLLALVPM GMPHTITRTT CFRGYTLPKG TEVFPLIGSI LHDPAVFQNP GEFHPGRFLD EDGRLRKHEA FLPYSLGKRV CLGEGLARAE LWLFFTSILQ AFSLETPCPP GDLSLKPAIS GLFNIPPDFQ LRVWPTGDQS R Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
CYP2S1
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
custom-made
CYP2S1
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
CYP2S1
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
CYP2S1
(Cytochrome P450, Family 2, Subfamily S, Polypeptide 1 (CYP2S1))
Cytochrome P450 2S1 (EC 1.14.14.-) (CYPIIS1) (Hydroperoxy icosatetraenoate dehydratase) (EC 4.2.1.152) (Thromboxane-A synthase) (EC 5.3.99.5),FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids. In epidermis, may contribute to the oxidative metabolism of all-trans-retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs). Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage. {ECO:0000250|UniProtKB:Q96SQ9}.