PPAP2C
(Phosphatidic Acid Phosphatase Type 2C (PPAP2C))
蛋白类型
Recombinant
产品特性
AA 1-276
宿主
小鼠
资源
HEK-293 Cells
标记
This PPAP2C protein is labelled with His tag.
原理
Predefined custom protein recombinant Plpp2 Protein expressed in mammalian cells.
序列
MERRWVFVLL DVLCVLVASL PFIILTLVNA PYKRGFYCGD DSIRYPYRPD TITHGLMAGV IITATVILVS LGEAYLVYTD RLYSRSNFNN YVAAIYKVLG TFLFGAAVSQ SLTDLAKYMI GRLRPSFLAV CDPDWSQVNC SGYVQLEVCR GSPANVTEAR LSFYSGHSSF GMYCMLFLAL YVQARLCWKW ARLLRPTVQF FLVAFAIYVG YTRVSDHKHH WSDVLVGLLQ GALVACLTVR YVSDFFKSRP PQPCQEDEVP ERKPSLSLTL TLGDRP Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Predefined custom protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
PPAP2C
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
WB, SDS, ELISA
custom-made
PPAP2C
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
WB, SDS, ELISA
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
PPAP2C
(Phosphatidic Acid Phosphatase Type 2C (PPAP2C))
Phospholipid phosphatase 2 (EC 3.1.3.-) (EC 3.1.3.4) (Lipid phosphate phosphohydrolase 2) (PAP2-gamma) (PAP2-G) (Phosphatidate phosphohydrolase type 2c) (Phosphatidic acid phosphatase 2c) (PAP-2c) (PAP2c),FUNCTION: Magnesium-independent phospholipid phosphatase that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P. Has no apparent extracellular phosphatase activity and therefore most probably acts intracellularly. Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound. Through dephosphorylation of these bioactive lipid mediators produces new bioactive compounds and may regulate signal transduction in different cellular processes (By similarity). Indirectly regulates, for instance, cell cycle G1/S phase transition through its phospholipid phosphatase activity (By similarity). {ECO:0000250|UniProtKB:O43688, ECO:0000250|UniProtKB:Q8K593}.